큰열매모자반(Sargassum macrocarpum)추출물의 나노에멀젼 제조공정 최적화 및 안정성평가
Optimization and Stability Evaluation of Manufacturing Process of Nanoemulsion Containing Sargassum macrocarpum Extracts
- 주제(키워드) Sargassum macrocarpum , Nanoemulsion , RSM , Optimization , Stability Evaluation
- 발행기관 부경대학교
- 지도교수 이양봉
- 발행년도 2021
- 학위수여년월 2021. 2
- 학위명 석사
- 학과 및 전공 대학원 식품공학과
- 원문페이지 ⅶ, 53
- 실제URI http://www.dcollection.net/handler/pknu/200000373957
- UCI I804:21031-200000373957
- 본문언어 한국어
- 저작권 부경대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Sargassum macrocarpum, known as a kind of seaweed, contains lots of functional compounds. Especially, the functional compounds such as sargahydroquinic acid, sargachromanol, and sargaquinoic acid are difficult to apply to food and medicinal products because they exhibit fat-soluble properties. The objective of this study is to optimize nanoemulsion with the extracts of Sargassum macrocarpum and to evaluate the stabilities of the optimized nanoemulsion as environmental factors of heat treatments and storage temperatures. The optimization process was investigated by selecting several factors that affect nanoemulsion manufacturing. They are Tween 80 concentration, stirring temperature, and stirring speed. In the design of response surface methodology(RSM), independent variables were Tween 80 concentration (X1, 2.5-4.5%, w/v), stirring temperature (X2, 28-61℃), and stirring speed (X3, 4,700-18,200rpm. Its dependent variables are particle size (Y1, nm), polydispersity index (Y2), and ζ-Potential (Y3, mV). The optimal condition obtained through RSM is 2.62% Tween 80, 47.1℃ stirring temperature, and 10,927 rpm stirring speed. In stability tests of nanoemulsion on heat treatment at 60, 100, 120°C, all the samples were relatively stable. The nanoemulsion samples during their storage at 4°C and 25°C for 28 days show relatively good stability. Optimized process and stability test of the nanoemulsion containing extracts of Sargassum macrocarpum show a good Potential to apply it to functional food products and medicines.
more목차
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 5
1.실험재료 5
2.실험방법 5
2.1.나노에멀젼 제조 5
2.2.실험설계 6
2.3.나노에멀젼 물리적 특성 9
2.3.1.입자크기 및 다분자지수(Polydispersity index) 9
2.3.2.제타전위(ζ-Potential) 9
2.3.3.미세구조 분석 9
2.4.나노에멀젼의 안정성 평가 10
2.4.1.열처리에 따른 안정성 평가 10
2.4.2.저장 안정성 평가 10
2.5.Sargahydroquinoic acid, sargachromenol와 sargaquinoic acid 함량 분석 10
2.6.통계처리 12
Ⅲ. 결과 및 고찰 13
1. 반응표면의 적합성 판단 13
2. 반응표면분석 19
2.1.입자크기 19
2.2.다분자지수(Polydispersity index) 21
2.3.제타전위(ζ-Potential) 22
2.4 최적 제조 조건 예측 및 검증 25
3. 미세구조 분석 27
4. MES-NE 안정성 평가 29
4.1.열처리에 따른 안정성 평가 29
4.2.저장 안정성 평가 33
4.3.Sargahydroquionoic acid, sargachromenol와 sargaquinoic acid 함량 분석 38
Ⅳ. 결론 40
Ⅴ. 참고문헌 41
Ⅵ. 감사의 글 52